First appearance in red and white for Patrik Hellebrand and Oscard Dorley.
Midfielder Jakub Křišťan and defender Daniel Kosek from Slavia reserves both played 45 minutes.
Yusuf fired wide and Zelený hit post in the biggest Slavia chances of the opening half.
Stanciu was denied by Ústí goalkeeper with his free kick in the second half. After 77 minutes the same player hit woodwork.
Ústí broke the deadlock after 79 minutes when Michal Bílek fired the ball through Markovič from the right side of the box.
Slavia play their second friendly game against Dynamo České Budějovice on Saturday. KO at 1:30 pm at Evžena Rošického stadium in Prague.
I. poločas: Kovář – Holeš, Frydrych, Zelený, Bořil – Křišťan, Traoré, Hellebrand, Oscar, Provod – Júsuf II. poločas: Markovič – Coufal, Kúdela, Takács, Kosek – Masopust, Ševčík, Souček, Stanciu, Olayinka – Tecl
Plachý – Pimpara (73. Hudec), Krátký, Písačka (46. Prošek), Drame (56. Jirkovský), Peterka, Bassey, Kolařík, Vlček, Vais, Valenta